PKCe is a member of the novel protein kinase C family of serine/threonine kinases.
Its activity is calcium-independent and phospholipid-dependent and is expressed at low levels in normal tissue except the brain, where expression is higher.
PKCe expression appears to be induced by interferon-a and its over-expression may play a role in tumorigenesis.
PKCe, through its regulatory domain, can induce immature neurite-like processes via a mechanism that appears to be of importance for neurite outgrowth during neuronal differentiation.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detects PKCe in Jurkat cell lysate.
Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P9103-20C PKCe (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by researcher.
Included Positive Antigen Control: (J9000)Jurkat cell lysate.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes mouse PKCe, Mr 95kD. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide, aa726-737 (KGFSYFGEDLMP), corresponding to the C-terminal variable (V5) region of mouse PKCe (KLH). Species sequence homology: rabbit.
