Metabotropic glutamate receptors have been classified into three groups.
In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission.
Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC).
Class II and class III mGluRs localized to the presynaptic membrane and are negatively coupled to adenylate cyclase.
It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: detects mGluR2/3 in 20ug of rat brain microsomal preparation.
Note: Do not boil the microsomal preparation, heat to 37°C for 15-30 minutes.
20ug of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M3884-76 (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Immunoprecipitation: 4ug immunoprecipitates mGluR2/3 from 400ug of rat brain microsomal preparation
Immunohistochemistry (paraffin): Detects mGluR 2/3 in paraffin embedded rat brain tissue sections
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes mGluR2 and mGluR3, MW: 100-110kD. Species Crossreactivity: rat and mouse. Does not crossreact with rabbit.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide (KQFVPTVCNGREVVDSTTSSL) corresponding to aa853-872 of human metabotropic glutamate receptor 2, with a lysine added to the N-terminus for conjugation (KLH).
