84-3353-71 [取扱停止]Pyk2 (CAKbeta, cdk-activating Protein Kinase beta) (Biotin) 100ul P9400-Biotin

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冷凍

特徴

  • Pyk2 is a protein tyrosine kinase related to Focal Adhesion Kinase (p125 FAK).
  • Pyk2 is highly expressed in the central nervous system.
  • It is phosphorylated on tyrosine residues in response to various stimuli that elevate intracellular calcium concentration, as well as by protein kinase C activation.
  • Applications:Suitable for use in Western Blot and Immunoprecipitation.
  • Other applications have not been tested.
  • Recommended Dilutions:Western Blot: 0.5-2ug/ml detected Pyk2 in mouse brain cytosolic RIPA lysates.
  • Lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P9400.
  • Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
  • Pyk2 has a MW of ~116kD as calculated from the amino acid sequence deduced from the complete human cDNA.
  • Immunoprecipitation: 4ug detected Pyk2 from 500ug of mouse brain cytosolic RIPA lysate
  • Optimal dilutions to be determined by the researcher.
  • Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
  • Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
  • For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
  • Aliquots are stable at -20°C for 12 months after receipt.
  • Dilute required amount only prior to immediate use.
  • Further dilutions can be made in assay buffer.
  • For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
  • Note: Applications are based on unconjugated antibody.

仕様

  • Size:100ul
  • Host:rabbit
  • Source Antibody:human
  • Grade:Affinity Purified
  • Purity:Purified by Protein A affinity chromatography.
  • Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
  • Specificity:Recognizes Pyk2 at 116kD in mouse brain cytosol. Also detects a band at 40kD, which may be a degradation product. Does not crossreact with FAK. Species Crossreactivity: human and mouse
  • Isotype:IgG
  • Calc Applications Abbrev:IP WB
  • Calc Crossreactivity:Hu Mo
  • Immunogen:GST fusion protein containing aa726-863 of human Pyk2
  • Shelf Life:1year
  • Swiss Prot Number:Q14289
  • EU Commodity Code:30021010
  • この商品は法規制を確認しておりません。(法規制によって販売できない場合もございます)
  • 製品の仕様は予告なく変更になる場合がございます。最新仕様はメーカーホームページをご確認ください。
  • 【試薬に関するお問合せ】
  • アズワン株式会社 試薬・プロセス材料グループ
  • TEL:06-6447-8641
  • FAX:06-6447-8642
  • E-mail:[email protected]
アズワン品番
84-3353-71
型番
P9400-Biotin
入り数
1個
標準価格
280,000円(税抜)
WEB価格
取扱停止
アズワン在庫 [?]
数量

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