84-3353-69　Kip2, p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) (Biotin) 100ul　K1851-Biotin

特徴

• The eukaryotic cell cycle is driven by the sequential action of a family of serine/threonine kinases, the cyclin dependent kinases (cdks) acting in concert with their regulatory subunits, the cyclins.
• p57 Kip2 is a potent inhibitor of several G1 cyclin/cdk2 complexes but unlike p27 Kip1 is not regulated by p53.
• Kip2 is thought to be involved in decisions to exit the cell cycle during development and differentiation.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2ug/ml detects Kip2(~57kD) in a HeLa nuclear extract and a bovine brain nuclear extract
• Immunoprecipitation: 5ug immunoprecipitates Kip2 from a HeLa nuclear extract and a bovine brain nuclear extract
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
• Positive Antigen Control Included:H1838: HeLa Nuclear Extract
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.