Two types of PDGF receptors have been identified: PDGF receptor A (PDGFRa) and PDGF receptor B (PDGFRb).
The PDGFRa, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGFRb, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity.
Both receptors are transmembranous, ligand-activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains.
The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Positive Antigen Control Included:F4209: 3T3/A31 cell lysate.
Use 20ug/lane for minigels
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human Platelet Derived Growth Factor Receptor Type B at ~190kD. Species Crossreactivity: mouse and monkey
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide corresponding to aa1095-1106, PAPRAEAEDSFL of human Platelet Derived Growth Factor Receptor Type B.
