Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an enzyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides.
Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis.
Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C.
elegans.
Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20kD (p20) and a 10kD (p10) subunit.
The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD).
Caspase-1 is highly expressed in spleen and lung, and is absent from brain.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 5ug/ml.
Immunocytochemistry (paraffin): 1:1000 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x RbI1904-40D: IgG, H&L (FITC) Pab Gt x RbI1904-46N: IgG, H&L (FITC) Pab Gt x RbI1904-41: IgG, X-Adsorbed (FITC) Pab Gt x Rb
Positive Control: THP cell lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes the p20 subunit of Caspase 1 and recognizes the P45 proenzyme (~45kD) and the p20 subunit (~20kD) of the reduced, human Caspase 1 isoform. May cross-react with Caspase 5 due to 92% sequence homology. Species sequence homology: mouse, rat, canine, porcine and feline (92% sequence homology).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the p20 subunit of human Caspase 1 conjugated with KLH.
