84-3352-71　NR1, phosphorylated (Ser897) (N-methyl-D-aspartate Receptor subunit NR1, GRIN1, NMDA1, NMDAR1, NMD-R1, Glutamate receptor ionotropic N-methyl D-aspartate 1) (APC) 100ul　N5380-11-APC

特徴

• NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor.
• The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate.
• As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor.
• A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium.
• Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations.
• The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell.
• Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml detected NR1 in 10ug of rat brain microsomal tissue lysate.
• Immunohistochemistry: 1:50 detected NR1 in normal rat hippocampus
• Immunoprecipitation: 10ug from a previous lot precipitated NR1 from rat brain microsomal RIPA lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
• Note: Applications are based on unconjugated antibody.