FYB (FYN binding protein) was initially identified as a molecular adaptor that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion.
TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of FYB, ultimately activates the NFkB family of transcription factors.
More recent experiments have shown that FYB controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bclx(L), suggesting that FYB regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation.
At least three isoforms of FYB are known to exist.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2.0ug/ml detected ADAP/SLAP-130/Fyb in RIPA lysates from RAW 264.7 macrophage cells.
RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-43.
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
Immunoprecipitation: 4ug immunoprecipitated ADAP/SLAP-130/Fyb from 500ug of RAW 264.7 macrophage RIPA lysate
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-40J: IgG, Fc (HRP) Pab Gt x Rb
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa755-768, DNDGEIYDDIADGC, of mouse Fyn binding protein (ADAP/SLAP-130/Fyb), conjugated to KLH. Species Sequence Homology: human and rat.
