84-3350-13　FADD (Fas-Associated Death Domain Protein, MORT1) (APC) 100ul　F0019-52-APC

特徴

• FADD was originally isolated as a protein that bound to the cytoplasmic domain of Fas in the yeast two-hybrid system.
• Sequence analysis revealed a region homologous to the death domain of Fas and TNFR-1.
• Subsequent biochemical studies have shown that FADD associates with Fas through interaction of the death domains.
• When over expressed in several cell lines, FADD induces apoptosis, which can be blocked by CrmA, an inhibitor of Caspase 1 (ICE).
• This evidence suggests that FADD plays a role in Fas-mediated apoptosis.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilution:Western Blot Analysis: 1-2ug/ml detects FADD in RIPA lysates from Jurkat cells.
• Jurkat cell lysate was resolved by electro-phoresis, transferred to nitrocellulose and probed with F0019-52 (1ug/ml).
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunoprecipitation: 4ug immunoprecipitates FADD from 500ug of Jurkat cell lysate
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control: J9000Jurkat lysate.
• Use 20ug per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C or colder.
• Aliquots are stable for at least 24 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.