Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an enzyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides.
Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis.
Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C.
elegans.
Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20kD (p20) and a 10kD (p10) subunit.
The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD).
Caspase-1 is highly expressed in spleen and lung, and is absent from brain.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 5ug/ml.
Immunocytochemistry (paraffin): 1:1000 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x RbI1904-40D: IgG, H&L (FITC) Pab Gt x RbI1904-46N: IgG, H&L (FITC) Pab Gt x RbI1904-41: IgG, X-Adsorbed (FITC) Pab Gt x Rb
Positive Control: THP cell lysate
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes the p20 subunit of Caspase 1 and recognizes the P45 proenzyme (~45kD) and the p20 subunit (~20kD) of the reduced, human Caspase 1 isoform. May cross-react with Caspase 5 due to 92% sequence homology. Species sequence homology: mouse, rat, canine, porcine and feline (92% sequence homology).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the p20 subunit of human Caspase 1 conjugated with KLH.
