Tbr2 (a T-domain transcription factor) is a member of a conserved protein family that shares a common DNA-binding domain, the T-box.
T-box genes encode transcription factors involved in the regulation of developmental processes.
Trb2 has been shown to play a role in the development of the neocortex and functions in the differentiation pathway of radial glia to postmitotic projection neurons.
Applications: Suitable for use in Western Blot and Immunohistochemistry
Other applications not tested.
Recommended Dilutions:Western Blot: 0.05ug/ml detected Tbr2 in 10ug of E13-14 mouse brain lysates
Immunohistochemistry (Paraffin): 1:1000 detected Tbr2 in mouse and human brain.
Requires HIER with citrate buffer pH 6.0Optimal dilutions to be determined by the researcher.
Positive Control:E13-14 mouse brain lysate
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes mouse Tbr2 observed at ~85/95kD. Target bands appear larger than the calculated molecular size of 74.8kD (isoform 1) and 72.7kD (isoform 2). Species Crossreactivity: human and rat
Isotype：IgY
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Linear peptide corresponding to mouse EOMES, conjugated to KLH. Epitope: C-Terminus.
