Nicotinic acetylcholine receptors, or nAChRs, are cholinergic receptors that form ligand-gated ion channels in the plasma membranes of certain neurons.
nAChR is triggered by the binding of the neurotransmitter acetylcholine (ACh) and is also opened by nicotine.
After binding ACh, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
nAChRs are present in many tissues in the body: the neuronal receptors are present in the central nervous system and the peripheral nervous system; and the neuromuscular receptors are found in the neuromuscular junctions of somatic muscles.
Neuronal AChR is composed of two different types of subunits: alpha (a) and beta (b).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Rat e16 brain lysate
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes rat Nicotinic Acetylcholine Receptor a4. Species Crossreactivity: mouse, human.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide selected from residues within the Nicotinic Acetylcholine Receptor a4. Epitope: Extracellular Domain
