Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development.
Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer.
Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3.
Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality.
Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1.
Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.
Applications:Suitable for use in ELISA, Western Blot, and Dot Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Western Blot: 1:250Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human Dnmt1, phosphorylated on Ser714.
Isotype：IgG
Calc Applications Abbrev：DB E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to aa residues surrounding Ser714 of human Dnmt1. Cellular Localization: Nucleus.
