he remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development.
Angiopoietin-1 is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor.
It is primarily expressed in developing endothelial cells.
During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2.
Ang-1 appears to play a crucial role in mediating matrix and mesenchyme.
It mediates blood vessel maturation and stability.
Ang-1 may play a critical role in heart development.
Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2.
A homolog of Ang-1, termed Angiopoietin-2 may act as an antagonist for Ang-1 and Tie-2.
Ang-1 and Ang-2 have ~60% sequence homology.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes mouse Angiopoietin-1. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide selected from mouse Angiopoietin-1 (KLH). Cellular Localization: Secreted. Species sequence homology: bovine, canine, porcine, equine (95%).
