Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500 detected PP1 beta in 10ug of HeLa lysates, Jurkat lysates, RAW 264 lysates, HEK293 lysates, NIH/3T3 lysates, C2C12 lysates, PC12 lysates, A431 lysates and HepG2 lysates.
Immunohistochemistry (Paraffin): 1:100 detected PP1 beta in normal human testis.
Tissue was pretreated with citrate buffer, pH 6.0.
Immunoprecipitation: 10-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human PP1 beta at ~38kD. Does not cross react with other recombinant PP1 isoforms. Species Crossreactivity: Mouse, Rat and Bovine
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Purified peptide corresponding to aa318-327 from the C-terminus of human PP1 beta catalytic subunit, conjugated to KLH.
