GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors.
The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation.
Gi belongs to the pertussis toxin sensitive G-proteins.
Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Paraffin sections require HIER with citrate buffer, pH 6.0Optimal dilutions to be determined by the researcher.
Positive Control:Western Blot: L6 Cell LysateImmunohistochemistry: Rat cerebellum
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human G protein subunit alphai1 and alpha2. Species Crossreactivity: mouse, rat and bovine
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa345-354 of Gi alpha1 and aa346-355 of Gi alpha2.
