84-3347-98 [取扱停止]GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule) (AP) 100ul G8959-11-AP
84-3347-98 [取扱停止]GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule) (AP) 100ul G8959-11-AP
This gene encodes a member of the GRB2/Sem5/Drk family.
This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling.
Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains.
This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains.
Transcript variants utilizing alternative polyA sites exist.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/ml detects GRAP2 from a Jurkat RIPA cell lysate
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:J9000 Jurkat Cell Lysate.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Use 20ug per lane for minigels.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity:Recognizes GADS, Mr 40kD. Species Crossreactivity: human and mouse.
Isotype:IgG
Calc Applications Abbrev:IP WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide corresponding to aa174-188 of mouse GADS (GEEIRPSVNRKLSDH) (KLH). Species sequence homology: human (14/15)