Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates
Immunoprecipitation: 5ul immunoprecipitated active Akt3/PKBg from 15nM IGF-I stimulated HEK293 cell lysates.
The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg)
Optimal dilutions to be determined by the researcher.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human Akt3/PKBg, Mr 60kD. An additional protein was observed, Mr 50kD. Detection of both bands is inhibited by preincubation with the immunizing peptide. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa119-131, CSPTSQIDNIGEE of human Akt3/PKBg.
