84-3347-89　［取扱停止］JAK2, phosphorylated (Janus Kinase 2) (Y1007, Y1008) (AP) 100ul　J0901-10A-AP

特徴

• The Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors.
• This association that results in tyrosine phosphorylation and activation.
• JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members.
• Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes.
• Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling.
• JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase.
• Different cytokines can activate via phosphorylation distinct JAK family members.
• In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2.0ug/ml.
• Detects phospho JAK2 in RIPA lysates from HEK293 cells expressing co-transfected JAK2 and chimeric Epo-Leptin receptors, stimulated with Epo.
• Phospho JAK 2 ~130kD
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.