84-3347-85　［取扱停止］MAP Kinase Kinase 7, phosphorylated (Thr275/Ser277) (Dual Specificity Mitogen-activated Protein Kinase Kinase 7, MAP2K7, MAPKK 7, MAPKK7, MKK7, PRKMK7, c-Jun N-terminal Kinase Kinase 2, JNK Kinase 2, JNKK 2, JNKK2, JNK-activating Kinase 2, MAPK/ERK Kinase 7, MEK 7, MEK7, Stress-activated Protein Kinase Kinase 4, SAPK Kinase 4, SAPKK4, SAPKK-4, SKK4) (AP) 100ul　M2363-95D-AP

特徴

• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 detects recombinant MKK7 1 that had been phosphorylated by MEKK1.
• Recombinant MKK7 1 was left untreated or phosphorylated by MEKK1 and resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution).
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.