FYB (FYN binding protein) was initially identified as a molecular adaptor that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion.
TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of FYB, ultimately activates the NFkB family of transcription factors.
More recent experiments have shown that FYB controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bclx(L), suggesting that FYB regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation.
At least three isoforms of FYB are known to exist.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2.0ug/ml detected ADAP/SLAP-130/Fyb in RIPA lysates from RAW 264.7 macrophage cells.
RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-43.
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
Immunoprecipitation: 4ug immunoprecipitated ADAP/SLAP-130/Fyb from 500ug of RAW 264.7 macrophage RIPA lysate
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-40J: IgG, Fc (HRP) Pab Gt x Rb
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes mouse ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa755-768, DNDGEIYDDIADGC, of mouse Fyn binding protein (ADAP/SLAP-130/Fyb), conjugated to KLH. Species Sequence Homology: human and rat.
