84-3347-79　［取扱停止］ATH 1 (MATH 1, NeuroD Family, Helix-loop-helix Transcription Factor) (AP) 100ul　A3950-01-AP

特徴

• ATH 1 (MATH 1, NeuroD Family) belongs to the basic helix-loop-helix (BHLH) family of transcription factors.
• It activates E-box dependent transcription.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:A3950-01A
• IMMUNOHISTOCHEMISTRY Protocol:1.
• Dissect tissues and freeze on dry ice
• 2.
• Cut on a cryostat -10um slices
• 3.
• Dry slides at RT for 30-90 min.
• 4.
• Fix with cold Acetone/Methanol (50/50) for 2 min
• Either process further or store at -20°C.
• Alternative is fixation with 4% PFA and treatment with a trypsin solution.
• (0.05% trypsin, incubation for 10 minutes at 37°C, followed by 3 washes with PBS)
• 5.
• Let air dry
• 6.
• Treat with PBS for 5 min
• 7.
• Incubate in 50mM ammonium chloride for 30 min
• 8.
• Treat with PBS for 5 min
• 9.
• Treat with Blocking serum for 30-45 min
• 10.
• Treat with A3950-01 for 90 min
• 11.
• Wash 3 times for 5 min
• 12.
• Secondary fluorescent conjugate antibody for 30 min
• 13.
• Wash 3 times for 5 min and mount.
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.