Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: detects STAT5B (doublet of 90kD and 92kD) in human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure
Immunoprecipitation: immunoprecipitates STAT5B from A431 carcinoma cells and a previous lot immunoprecipitated STAT5B from human Raji cells.
A RIPA lysate of human A431 cells (500ug protein) was incubated with 4ug of anti-STAT 5B Immunecomplexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B.
Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection
Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes the 90-92kD forms of human STAT5B protein. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：aa774-787 of human STAT5B (CGRPMDSQWIPHAQS)
