Not suitable for Immunoprecipitation on native PME-1.
Other applications not tested.
Recommended Dilution:Western Blot: detects PME-1 in RIPA lysates from mouse 3T3 fibroblasts.
3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4290-05 (2ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemi-luminescence detection system
Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human Protein Phosphatase Methylesterase-1, Mr 44kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A mixture of two nickel agarose-purified, His6-tagged, bacterial expressed human Protein Phosphatase Methylesterase-1 (PME-1) fragments (residues 225-302 and 277-387). These two fragmentsrepresent the carboxyl-terminal portion of the protein.
