NADPH Oxidase is comprised of flavocytochrome b558 (made up of p22-phox and gp91-phox), p40-phox, p47-phox and p67-phox.
NADPH Oxidase mediates the final steps of electron transfer to oxygen, resulting in the generation of superoxide ion.
A growing body of evidence suggests a potential role for oxygen-derived radicals such as superoxide anions and hydrogen peroxide as intracellular signaling molecules that may be involved in cell growth, apoptosis and cellular senescence.
Applications:Suitable for use in Western Blot.
Not recommended for Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-4ug/ml detects gp91-phox in a HL60 membrane, RIPA cell lysate and human neutrophil membranes.
HL60 membrane lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8570.
Proteins were visualized using a secondary antibody IgG (HRP) Pab Gt x Rb* and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
*Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Positive Control:Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human gp91-phox, Mr 75kD. Depending on the type of lysate, the amount loaded, the gel and the blot conditions, gp91-phox may run as a sharp band, Mr ~75kD, or appear as a smear between 75-91kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa548-560, KQSISNSESGPRG, of human gp91-phox (KLH). Species Sequence Homology: mouse-100%; bovine-11/13; porcine-10/13.
