Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells.
Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue
Immunocytochemistry: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutesImmunohistochemistry: Reported to detect CoREST in tissue sections.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human CoREST, Mr ~66kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：GST fusion protein corresponding to residues 109-293 of human CoREST.
