S-Adenosyl-I-methionine-dependent protein arginine N-methyltransferases (PRMTs) methylate arginine residues within a variety of proteins, particularly histones.
At least four distinct mammalian family members have now been described, including PRMT1, PRMT3, CARM1/PRMT4 and JBP1/PRMT5
Applications:Suitable for use in Western Blotting, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: Reacts with a band of approximately 57kD on whole HL60 cell lysate.
Additional bands may be present depending on sample used
ELISA (direct): 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified immunoglobulin
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity:Recognizes human PRMT3 (Protein Arginine N-Methytransferase 3).
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide from N-terminus of human PRMT3.