TIMP-1and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity.
TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B).
TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 5-10ug/ml.
Recognizes a band at ~28-30kD in reduced samples
Immunohistochemistry: 1ug/sectionELISA: 5ug/mlOptimal dilution to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human TIMP (Tissue Inhibitor of Metalloproteinase). Recognizes native and denatured TIMP and TIMP in and out of enzyme/inhibitor complex. Does not crossreact with other human proteins tested. Not known to inhibit biological activity. Species Crossreactivity: rabbit, sheep, bovine, rat, equine and ferret
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Eq Hu Rb Rt Sh
Immunogen：Full length purified human fibroblast TIMP (Tissue Inhibitor of Metalloproteinase).
