Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes.
To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag.
The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag.
Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:10,000–1:30,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：chicken
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes red fluorescent protein (RFP). Shows no reactivity to GFP.
Isotype：IgY
Calc Applications Abbrev：WB
Immunogen：Recombinant red fluorescent protein (RFP), highly purified by affinity chromatography on Nickel column.
