Applications:Suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Immunohistochemistry Protocol:1.
Dissect tissues.
Freeze on dry ice
2.
Cut on a cryostat ~10um slices
3.
Dry slides at RT for 30-90 minutes
4.
Fix with cold Acetone/Methanol (50/50) for 2 min then either process further or store at -20°C.
Alternative is fixation with 4% PFA and treatment with a trypsin solution (0.05% trypsin, incubation for 10 min.
at 37°C, followed by 3 washes with PBS)
5.
Let air dry
6.
Treat with PBS for 5 minutes
7.
Incubate in 50mM ammonium chloride for 30 minutes
8.
Treat with PBS for 5 minutes
9.
Incubate with Blocking Serum for 30-45 minutes
10.
Incubate with appropriately diluted L2075 for 90 minutes
11.
Wash 3 times for 5 minutes
12.
Incubate with fluorescent conjugated secondary anti-rabbit IgG for 30 minutes
13.
Wash 3 times for five minutes.
Mount.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes the human Lim homeobox containing transcription factor LHX2. No significant sequence homology with other proteins. Species Crossreactivity: rodent.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Rd
Immunogen：Synthetic peptide corresponding to aa239-254 af human LHX2/Lim2.
