Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunoblot: 1:2500-1:5000 ELISA: 1:2000-1:4000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes most mammalian laminins and has negligible crossreactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific crossreaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
