c-fos is a 55kD nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents.
c-fos is also induced in neuronal tissue following drug induced seizures.
Applications:Suiatble for use in Western Blot and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein.
Detects a band at 55-60kD on immunoblots of stimulated HeLa and HL-60 lysates.
Immunocytochemistry: Has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human c-fos. Species Crossreactivity: Rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala- Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.
