The catalytic domain of serum and glucocorticoid-regulated protein kinase (SGK) is 54% identical with that of Akt/PKB.
Although SGK1 lacks the PtdIns(3,4,5) P3-binding Pleckstrin-homology domain, it retains the residues that are phosphorylated by PDK1 (Thr256 and Ser422).
PDK1 activates SGK in vitro by phosphorylating Thr256.
Like Akt/PKB, SGK1 preferentially phosphorylates serine and threonine residues that lie in RXRXXS/T motifs.
SGK1 can be activated by PI-3 Kinase, which makes it an important signaling molecule in growth factor and insulin dependent signaling pathways.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot Analysis: 0.5-2ug/ml detects SGK1 in RIPA lysates from A431 and Rat 2 cells.
Rat 2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S1010-83 (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Immunoprecipitation: 4ug Immunoprecipitates SGK1 from 500ug of Rat 2 lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human SGK1, Mr ~50kD. A protein, competed by the peptide, was also detected in Rat 2 lysates, Mr ~31kD.Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to amino acids 399-412 (C-GKSPDSVLVTASVK) of human SGK1, KLH-conjugated. Species sequence homology: Rat and mouse 13/15aa identical
