84-3346-69　［取扱停止］Protein Phosphatase 2A, alpha (PP2Aa) (AP) 100ul　P9107-33B-AP

特徴

• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot Analysis: 0.5-2ug/ml dilution detects PP2A, A subunit in RIPA lysates from EGF-stimulated A431 cell lysate.
• NIH 3T3 cell lysate was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-PP2A, A subunit).
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Band is PP2A, A subunit (63- 65kD).
• Positive Antigen Control:F4209: Fibroblast Cell, 3T3/A31
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.