Applications:Suitable for use in ELISA and Western Blot.
Not suitable for use in Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-4ug/ml will detect Casein Kinase 2 in RIPA lysates from mouse 3T3 fibroblasts.
3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Casein Kinase 2 (4ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Positive Control for Immunoblot Analysis:3T3 cell lysate; use 20ug per lane for immunoblots.
Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil before use).
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human casein kinase 2 at 44kD and an unknown band at 66kD. Species Crossreactivity: Mouse. Predicted crossreactivity with rat, rabbit, chicken, frog and starfish.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ch Fr Hu Mo Rb Rt
Immunogen：Synthetic peptide (C-SRARVYTDVNTHRPRE) corresponding to aa7-22 of human Casein Kinase 2, KLH-conjugated.
