Metabotropic glutamate receptors have been classified into three groups.
In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission.
Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC).
Class II and class III mGluRs localized to the presynaptic membrane and are negatively coupled to adenylate cyclase.
It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: detects mGluR2/3 in 20ug of rat brain microsomal preparation.
Note: Do not boil the microsomal preparation, heat to 37°C for 15-30 minutes.
20ug of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M3884-76 (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Immunoprecipitation: 4ug immunoprecipitates mGluR2/3 from 400ug of rat brain microsomal preparation
Immunohistochemistry (paraffin): Detects mGluR 2/3 in paraffin embedded rat brain tissue sections
Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes mGluR2 and mGluR3, MW: 100-110kD. Species Crossreactivity: rat and mouse. Does not crossreact with rabbit.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide (KQFVPTVCNGREVVDSTTSSL) corresponding to aa853-872 of human metabotropic glutamate receptor 2, with a lysine added to the N-terminus for conjugation (KLH).
