Calreticulin was originally identified as a Ca2+ binding protein in the muscle sarcoplasmic reticulum.
Since its original discovery, it has been found in high abundance in non-muscle tissue.
Calreticulin has the ability to bind Ca2+ with high capacity but low affinity.
The exact function of calreticulin is not yet known, however recent reports suggest that it may play a role in nuclear hormone receptor gene transcription and may also act as a chaperone protein.
Calreticulin is 90% conserved among human, mouse, rat, rabbit and Aplysia.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Detects calreticulin in tissue sections and cells.
Optimal diltions to be determined by the researcher.
Included Positive Antigen Control:F4209: Fibroblast Cell, 3T3/A31 lysate.
Add 2.5ul 2-mercaptoethanol/100ul of lysate.
Boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for Western Blot analysis.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human calreticulin. Does not crossreact with other endoplasmic reticulum proteins. Species Crossreacitivity: mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt Xe
Immunogen：Synthetic peptide corresponding aa412-417, QAKDEL, at the C-terminus of human calreticulin, conjugated to KLH.
