Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.5-2.0ug/ml detects MEK1 in RIPA lysates from A431 and 3T3/A31 cells.
A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2865-07 (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
MEK1 (~45kD).
Immunoprecipitation: 4ug immunoprecipitates MEK1 from 500ug of A431 and 3T3/A31 RIPA lysate.
MEK1 Immunoprecipitation Kinase Assay, nonradioactive:4ug of this lot immunoprecipitated active MEK1 from NGF stimulated PC-12 cell lysates, as well as active human MEK1
Optimal dilutions to be determined by the researcher.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes mouse MEK1 at 45kD. Species Crossreactivity: Human, mouse and rat
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa2-18 (CPKKKPTPIQLNPAPDGS) of mouse Map Kinase Kinase (MEK1)(KLH). Species sequence homology: rat, mouse, rabbit-100%; Chinese hamster and chicken-16/17.
