MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
AMPK is a heterotrimeric complex comprising a catalytic alpha subunit and regulatory beta and y subunits.
It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.
Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.
The human AMPKalpha1 and AMPKalpha2 genes encode 548 amino acid and 552 amino acid proteins, respectively.
Human AMPKbeta1 encodes a 271 amino acid protein and human AMPKbeta2 encodes a 272 amino acid protein.
The human AMPKy1 gene encodes a 331 amino acid protein.
Human AMPKy2 and AMPKy3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Dye/Protein Ratio: 2.16
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes endogenous levels of human AMPKalpha1 only when phosphorylated at Thr183 and human AMPKalpha2 only when phosphorylated at Thr172. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human AMPKalpha1 around the phosphorylation site of Threonine 183.
