84-3346-09　［取扱停止］SOS2 (Son of sevenless Homolog 2, SOS-2) (HRP) 100ul　225971-HRP

特徴

• The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities.
• Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules.
• Ras-GRF p140 promotes nucleotide exchange on Ras p21s but not on other members of the Ras gene superfamily.
• In addition, three mammalian homologs of the Drosophila Ras-GRF, son of sevenless (Sos), have been described.
• These include two from mouse, mSos 1 and mSos 2, and one from human, hSos.
• Vav p95 has been reported to function as a GRF in activation of Ras by the T cell receptor and has been reported to have a domain similar to that of Dbl p115, which is a GRF specific for Cdc42Hs.
• Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.