Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes.
TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins.
These antiviral proteins are found in different cell compartments; TLRs (i.e.
TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm.
Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection.
Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes endogenous levels of human IFIH1.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human IFIH1 (Q9BYX4).
Shelf Life：1year
Swiss Prot Number：Q9BYX4
EU Commodity Code：30021010
商品名：IFIH1 (Interferon-induced Helicase C Domain-Containing Protein 1, Clinically amyopathic dermatomyositis Autoantigen 140kD, CADM-140 Autoantigen, Helicase with 2 CARD Domains, Helicard, Interferon-induced with Helicase C Domain Protein 1, Melanoma Differentiation-Associated Protein 5, MDA-5, Murabutide down-Regulated Protein, RIG-I-like Receptor 2, RLR-2, RNA Helicase-DEAD box Protein 116, IFIH1, MDA5, RH116) (Biotin) 100ul
