MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM).
An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors.
TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.
Applications:Suitable for use in Flow Cytometry (Not Tested), Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Not Tested): 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlWestern Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:Placenta or breast carcinoma
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human TIMP3. Species Crossreactivity: mouse, rat, bovine, canine and equine.
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Bo Ca Eq Hu Mo Rt
Immunogen：Peptide corresponding to aa175-211 from TIMP3. Species Sequence Homology: mouse, rat
