MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.
There are two closely related napsins, napsin A and napsin B.
Napsin A is expressed as a single chain protein.
Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen.
Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung.
The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Applications:Suitable for use in Flow Cytometry (Not Tested), Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Not Tested): 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlWestern Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:Lung adenocarcinoma
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human Napsin-A.
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide RFDPKASSSFQANGTKFAIQYGT corresponding to human Napsin-A.
