MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Nerve growth factor-beta (NGF-b) is a potent neurotrophic factor which supports the growth and survivability of nerve and/or glial cells.
The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, formed by two 13.5kD polypeptide monomers (238 total aa).
The three disulfide bonds are required for biological activity.
Applications:Suitable for use in FLISA, Western Blot and Neutralization.
Other applications not tested
Recommended Dilution:FLISA: 0.5-2ug/ml.
This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF as a detection antibody, allows the detection of 0.2-0.4ng/well of recombinant Human beta-NGF
Western Blot: 0.1-0.2ug/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3ng/lane, under either reducing or non-reducing conditions
Neutralization: To yield one-half maximal inhibition of the biological activity of Human beta-NGF (1ng/ml), a concentration of 0.05-0.08ug/ml of this antibody is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
