MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
TNF RII (p75, CD120b) is a 75kD transmembrane glycoprotein originally isolated from a human lung fibroblast library.
Among the multitude of cells known to express TNFRII are monocytes, endothelial cells, Langerhans cells, and macrophages.
Mouse to human amino acid sequence identity in the TNFRII cytoplasmic domain is 73%, while aa sequence identity in the extracellular region falls to 58%.
This drop in extracellular identity is reflected in the observation that human TNF-alpha is not active in the mouse system.
TNF RII to TNF RI, amino acid sequence identity is only about 20% in the extracellular region and 5% in the cytoplasmic domain.
TNF RII consists of a 240aa residue extracellular region, a 27aa residue transmembrane segment and a 173aa residue cytoplasmic domain.
TNF R1 and TNF R2 are members of a family of structurally related membrane receptors that includes lymphotoxin receptor, Fas, WSL1, DR4, CD40, CD30, CD27, 4-1BB, OX40, and p75 nerve growth factor receptor.
Members of the TNFR family can interact through their cytoplasmic domains with a range of intracellular signalling proteins, most of which fall into two distinct groups.
The first is the death domain containing proteins, including TRADD, FADD/MORT1, and RIP, which associate directly with receptors also containing death domains, such as TNF R1 and Fas.
The second is the TRAF proteins.
TRAF1 and TRAF2 were originally identified by their association with the cytoplasmic domain of TNFR2.
TRAF proteins appear to function as adaptor proteins.
TRAF2 directly binds at least eight intracellular molecules, including TRAF1, cIAP1, cIAP2, I-TRAF/TANK, A20, TRIP, RIP, and NIK.
The best characterized TRAFmediated signal transduction pathway is the activation of NF-B transcription factors.
TRAF2 mediates NF-B activation via the recruitment of the serine/threonine kinase NIK, which can in turn activate CHUK, an IBspecific kinase that triggers IB degradation.
In addition to recruiting mediators of NF-B activation, TRAF2 can bind at least three other molecules (I-TRAF/TANK, A20, TRIP) that inhibit its ability to activate NF-B.
Applications:Suitable for use in FLISA, Western Blot, Immunohistochemistry and Neutralization.
Using in conjunction with T9162-72A, as a detection antibody, the detection limit of recombinant human sTNF Receptor II is ~0.2-0.4ng/well
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human sTNF-receptor II is 1.5-3ng/lane, under either reducing or non-reducing conditions
Immunohistochemistry: 3ug/ml incubated overnight at 4°C followed by a fluorescent labeled secondary antibody.
Stained PC-3 and CaCo-2 cells
Neutralization: 15-20ug/ml yields one-half maximal inhibition of the biological activity of Human sTNF Receptor-II (0.5ug/ml)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human sTNF Receptor Type II.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human sTNF Receptor Type II, expressed in E. coli.
