MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Three isoforms of nitric oxide synthase (NOS) have been identified.
All are homodimers with subunits of 130-160 kDa.
All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain).
The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site.
Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production.
NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO).
Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane.
In response to increased intracellular Ca2+, nNOS interacts with CaM.
The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm.
The dephosphorylation of nNOS by calcineurin initiates the production NO.
NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways.
nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).
Applications:Suitable for use in Dot Blot, FLISA
Recommended Dilution:FLISA: 1:1,000Dot blot 1:500
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：DB FLISA
Calc Crossreactivity：Hu
Immunogen：Phospho-nNOS-S1417 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1403~1432 amino acids surrounding S1417 of human nNOS.
