MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Maf acts as a transcriptional activator or repressor.
When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription.
Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Binds to the ARE sites of detoxifying enzyme gene promoters (By similarity).
Involved in embryonic lens fiber cell development.
Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation.
Activates the expression of IL4 in T helper 2 (Th2) cells.
Increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression.
Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element.
Activates transcription of the CD13 proximal promoter in endothelial cells.
Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction.
Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development.
Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter.
Binds to the T-MARE (Maf response element) sites of lens-specific alpha-and beta-crystallin gene promoters.
Binds element G1 on the glucagon promoter.
Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells.
It may interact with additional basic-zipper proteins that determine a subtype of Maf-responsive element binding.
Applications:Suitable for use in Western Blot and FLISA.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes mouse Maf.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 14-42 in the N-terminal region of mouse Maf, UniProt Accession #P54843. Species sequence homology: bovine, chicken, human, rat
