84-3283-74　［取扱停止］ATG7 (APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like, autophagy-related protein 7, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-activating enzyme E1-like protein) (MaxLight 750) 100ul　032233-ML750

特徴

• MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
• APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12.
• APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE).
• APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3.
• Formation of the PE conjugate is essential for autophagy.
• Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation.
• Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
• Applications:Suitable for use in Immunohistochemistry, FLISA
• Recommended Dilution:FLISA: 1:1,000Immunohistochemistry: 1:10-50
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™750 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.