MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Routinely used therapies are not adequate to treat the heterogeneity of breast cancer, and consequently, more therapeutic targets are desperately needed.
To identify novel targets, we generated a breast cancer cDNA library enriched for genes that encode membrane and secreted proteins.
From this library we identified SUSD2 (Sushi Domain Containing 2), which encodes an 822-amino acid protein containing a transmembrane domain and functional domains inherent to adhesion molecules.
Previous studies describe the mouse homolog, Susd2, but there are no studies on the human gene associated with breast cancer.
Immunohistochemical analysis of human breast tissues showed weak or no expression of SUSD2 in normal epithelial cells, with the endothelial lining of vessels staining positive for SUSD2.
However, staining was observed in pathologic breast lesions and in lobular and ductal carcinomas.
SUSD2 interacts with galectin-1 (Gal-1), a 14kD secreted protein that is synthesized by carcinoma cells and promotes tumor immune evasion, angiogenesis, and metastasis.
Interestingly, we found that localization of Gal-1 on the surface of cells is dependent on the presence of SUSD2.
Various phenotype assays indicate that SUSD2 increases the invasion of breast cancer cells and contributes to a potential immune evasion mechanism through induction of apoptosis of Jurkat T cells.
Using a syngeneic mouse model, we observed accelerated tumor formation and decreased survival in mice with tumors expressing Susd2.
We found significantly fewer CD4 tumor infiltrating lymphocytes in mice with tumors expressing Susd2.
Together, our findings provide evidence that SUSD2 may represent a promising therapeutic target for breast cancer.
Applications:Suitable for use in Western Blot, Immunohistochemistry and FLISA.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human SUSD2.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 716-745 in the C-terminal region of human SUSD2.
