84-3221-53 [取扱停止]Inositol 1,4,5-trisphosphate 3-kinase A, CT (Inositol-trisphosphate 3-kinase A, InsP 3-kinase A, IP3 3-kinase A, IP3K A, IP3KA, IP3-3KA, ITPKA) (MaxLight 750) 100ul I7655-65C-ML750
84-3221-53 [取扱停止]Inositol 1,4,5-trisphosphate 3-kinase A, CT (Inositol-trisphosphate 3-kinase A, InsP 3-kinase A, IP3 3-kinase A, IP3K A, IP3KA, IP3-3KA, ITPKA) (MaxLight 750) 100ul I7655-65C-ML750
MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
ITPKA regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4.
The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling.
Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.
It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.
ITPKA and ITPKB are 68% identical in the C-terminus region.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000 Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity:Recognizes human ITPKA. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide selected from the C-terminal region of human ITPKA (KLH).